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微信小章| 产品名称： | Rotavirus A |
|---|---|
| 商品货号： | TS191296 |
| Classification： | Reoviridae, Rotavirus, Rotavirus A |
| Agent： | Rotavirus A |
| Strain： | Hu/Australia/1-9-12/77/S |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | feces, human Melbourne Australia Isolation date: 1977 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects, minimal rounding and debris generation CPE, minimal rounding and debris generation. |
| Recommended Host： | Host of Choice: MA-104 (ATCC CRL-2378) Host Range: MA-104 |
| Growth Conditions： | Temperature: 37.0°C Duration: 3-4 days, rocking may be necessary to increase titer. |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | G Barnes, ATCC |
| Special Collection： | NCRR Contract |
| Source： | feces, human Melbourne Australia Isolation date: 1977 |
| Year of Origin： | 1977 |
| References： | Albert MJ, et al. Live attenuated human rotavirus vaccine. US Pat. 4,624,850 dated Nov 25, 1986. Other MA-104 cells should also work. child with gastroenteritis VR-2105 is contaminated with bacteria; VR-1546 is a cleaned version of this same strain. The virus must be grown in serum-free media containing trypsin and should be pre-treated with trypsin to activate the virus before inoculation. Cell monolayers must be washed before inoculation to remove any FBS that would inactivate the trypsin. VR-1546 created by freeze-thawing VR-2105 three times, then sterile-filtering it through 0.45 micron filter one time and 0.2 micron filter two times before inoculating. Sato K, et al. Isolation of human rotavirus in cell cultures: brief report.. Arch. Virol. 69: 155-160, 1981. PubMed: 6171239 |