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| 产品名称： | C225/U2-T1 C225/U2/T1, C225/U2-T1 |
|---|---|
| 商品货号： | TS191313 |
| Designations： | C225/U2-T1 C225/U2/T1, C225/U2-T1 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Applications： | A 2.0 kb BamHI fragment of the insert has been used to detect a mRNA of about 4.3 kb which was present at higher concentration in galactose-grown Saccharomyces. |
| Vector： | Construct size (kb): 13.69999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 6.0 Gene product: alpha-ketoglutarate dehydrogenase KGD1 |
| Insert Size (kb)： | 6.0 |
| Genotype： | MATalpha ura3-1 kgd1-1 |
| Mating Type： | alpha |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb)(approx): SstI- 0.5- HindIII- 0.4- BamHI- 0.9- HindIII- 1.2- BamHI- 1.3- EcoRI- 0.5- EcoRI. Restriction digests of the clone give the following sizes (kb): SmaI/SalI--7.0 (doublet); SalI--13.7; EcoRV--12.9, 0.8; XbaI--8.0, 5.7. A 2.0 kb BamHI fragment of the insert has been used to detect a mRNA of about 4.3 kb which was present at higher concentration in galactose-grown Saccharomyces. A regulatory element has been demonstrated between -354 and -143 (relative to +1 being the A of the ATG initiation signal). pG70/T1 is available in Escherichia coli RR1 (TS191313) and in Saccharomyces cerevisiae C225/U2 (ATCC 66320). |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Repetto B, Tzagoloff A. Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase. Mol. Cell. Biol. 9: 2695-2705, 1989. PubMed: 2503710 |