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微信小章| 产品名称： | pHN3491 |
|---|---|
| 商品货号： | TS191360 |
| Designations： | pHN3491 |
| GenBank Number： | M11710 |
| Species： | Rattus norvegicus, rat |
| Depositors： | CJ Lusty |
| Vector： | Construct size (kb): 8.239999771118164 |
| Insert： | DNA: cDNA Insert lengths(kb): 5.650000095367432 Tissue: liver Gene product: carbamoyl-phosphate synthetase 1, mitochondrial CPS1 |
| Insert Size (kb)： | 5.650 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--4.0, 3.2, 1.05; ClaI--8.25; HindIII--4.6, 3.7; PstI--4.0, 2.4, 1.0, 0.6, 0.3; BamHI--8.25. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.60, 3.0, 3.4; BamHI--0.72; HindIII--4.3; EcoRI--4.7. With prolonged culture on plates, the poly(A) tail becomes shorter. A full-length cDNA clone, with the coding sequence 140 - 4640 bp from the 5 end. A 4.0 kb fragment containing most of the coding sequence can be obtained by a BamHI/EcoRI double digest. The order of the major features in this plasmid is: HindIII - 273 bp SV40 linker - PstI - GC tail (approx. 15 bp) - 5 end - insert - 3 end - AT tail (approx. 110 bp) - 25 bp SV40 - PvuII - pBR322 fragment, including ampR, of 2295 bp. |
| References： | Lagace M, et al. Rat carbamyl-phosphate synthetase I gene. J. Biol. Chem. 262: 10415-10418, 1987. PubMed: 3038878 Nyunoya H, et al. Characterization and derivation of the gene coding for mitochondrial carbamyl phoshate synthetase I of rat. J. Biol. Chem. 260: 9346-9356, 1985. PubMed: 2991241 Nyunoya H, et al. The gene coding for carbamoyl-phosphate synthetase I was formed by fusion of an ancestral glutaminase gene and a synthetase gene. Proc. Natl. Acad. Sci. USA 82: 2244-2246, 1985. PubMed: 2986106 Carol J Lusty, personal communication |