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| 产品名称： | pSLF102 |
|---|---|
| 商品货号： | TS191404 |
| Designations： | pSLF102 |
| Depositors： | SL Forsburg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.4000000953674320 Vector: pSLF102 (plasmid) Promoters: Promoter CaMV Marker(s):ampR,ura4+ Construct size (kb): 7.400000095367432 Features: marker(s): ampR marker(s): ura4+ operator: tet promoter: CaMV replicon: ars1 replicon: pMB1 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.4; HindIII--5.6, 1.8; SmaI--7.4. The vector contains the constitutive CaMV promoter adjacent to the tet operator. The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+. A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516 |
| Related Products： | component of:ATCC 87685 |
| Shipped： | freeze-dried |