Construct size (kb): 5.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKM3
Other gene symbols: ERK1
Gene name: protein kinase, mitogen activated 3 (MAP kinase 3; p44)
Contains complete coding sequence?: N
Chromosome: 16; Localization: 16
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5 end: HindIII
Insert 3 end: NotI
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: T09258; EST: EST07151; Primer: M13 Reverse;
THC id: THC97562
DNA Seq. Acc.: X60188; EST: EST07151
Nucleotides 1-348 of the insert correspond to
nucleotides 158-505 of X60188 with 99% identity.

Insert lengths(kb): 1.799999952316284

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: mitogen activated protein kinase 3( p44), protein kinase, mitogen activated kinase 3, protein kinase, mitogen-activated 3 (MAP kinase 3) ERK1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): HindIII+NotI--3.2, 0.94, 0.78; EcoRI--5.0.

Based on the alignment with EMBL accession X60188 (1866 nt, CDS 73-1212), this clone appears to contain part of the coding region.

The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII.