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| 产品名称： | 2A3 |
|---|---|
| 商品货号： | TS191498 |
| Organism： | Homo sapiens, human |
| Tissue： | pharynx |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papillomavirus (HPV) DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 56 years old |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | (P16) hypodiploid to hypertriploid with modal number = 64 |
| Derivation： | The 2A3 cell line was derived by transfecting ATCC HTB-43 (FaDu) with E6 and E7 genes using PA317 LXSN 16E6/E7. The pLXSN16E6E7 vector contained the human papilloma virus (HPV) type 16 E6 and E7 genes under control of the Moloney murine leukemia virus (MoMuLV) promoter-enhancer sequences. The vector also contained a gene encoding resistance to neomycin transcribed from the SV40 promoter. |
| Clinical Data： | 56 years Caucasian male |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I) |
| Comments： | The 2A3 cell line was derived by transfecting ATCC HTB-43 (FaDu) with E6 and E7 genes using PA317 LXSN 16E6/E7. The pLXSN16E6E7 vector contained the human papilloma virus (HPV) type 16 E6 and E7 genes under control of the Moloney murine leukemia virus (MoMuLV) promoter-enhancer sequences. The vector also contained a gene encoding resistance to neomycin transcribed from the SV40 promoter. This stably transfected head and neck cell line may be used as a basis for animal models for drug development as it was 100% tumorigenic in nude mice while continuing to express E6 oncoprotein in vivo. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: None detected CSF1PO: 12 D13S317: 8, 9 D16S539: 11 D5S818: 12 D7S820: 11, 12 THO1: 8 TPOX: 11 vWA: 15, 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 2 PGM3, 1 |
| Population Doubling Time： | 51 hours |
| Name of Depositor： | E Dadachova, Ph.D. |
| Year of Origin： | June 2010 |
| References： | Harris M, et al. Radioimmunotherapy of experimental head and neck squamous cell carcinoma (HNSCC) with E6-specific antibody using a novel HPV-16 positive HNSCC cell line. Head Neck Oncol. 3(1): 9, 2011. PubMed: 21314983 |