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| 产品名称： | 5E10 PTA-865 |
|---|---|
| 商品货号： | TS191504 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Cancer |
| Age： | adult |
| Strain： | BALB/c |
| Applications： | Western blot analysis demonstrates the 5E10 antigen is a transmembrane glycoprotein that has a molecular weight of about 115 Kd. |
| Comments： | Animals were immunized with a mixture of six prostate tumor cell lines: primary cell lines, ALVA31, JCA1 and ND1; metastatic cell lines, DU145, LNCaP and PC3. 5E10 recognizes an antigenic determinant on the surface of normal and cancerous prostate cells but not on the surface of non-prostatic tumor cells, peripheral blood cells, bone marrow hematopoietic and stromal fibroblast cells, or endothelial cells. The epitope recognized by 5E10 is extracellular. Western blot analysis demonstrates the 5E10 antigen is a transmembrane glycoprotein that has a molecular weight of about 115 Kd. A culture deposited at the ATCC as PTA-865 in October of 1999 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cured cell line is available as CRL-2698. The original patent deposit is available as PTA-865. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5%
|
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 kappa |
| Name of Depositor： | Univ. Iowa Res. Fndn. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Weiner GJ, et al. Prostate cell surface antigen-specific antibodies. US Patent 6,207,805 dated Mar 27 2001 Rokhlin OW, et al. 5E10: a prostate-specific surface-reactive monoclonal antibody. Cancer Lett. 131: 129-136, 1998. PubMed: 9851244 |