微信小陈
微信小章
产品简介
购买须知
| 产品名称： | KS+SV2CAT plasmid in E. coli |
|---|---|
| 商品货号： | TS191614 |
| Designations： | KS+SV2CAT plasmid in E. coli |
| Depositors： | PA Tsonis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.40 Vector: KS+SV2CAT (plasmid) Promoters: Promoter T3 Construction: pSV2cat, pBluescript KS+ Marker(s):ampR Construct size (kb): 5.400000095367432 Features: marker(s): ampR promoter: SV40, T3, T7 replicon: pMB1 |
| Applications： | expression vector shuttle vector vector permitting RNA synthesis in vitro |
| Comments： | Additional polylinker sites are ApaI and KpnI. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 2.1; SmaI--4.7; PstI--4.7; EcoRI--4.l, 0.72; ClaI--4.7. Mammalian shuttle expression vector containing a CAT (chloramphenicol acetyltransferase) gene expressed under an SV40 promoter. To be used as a control for promoter activity in conjunction with the promoter cloning plasmid KS+SV0CAT (ATCC 37608). Constructed by inserting the AccI-ApaI fragment of pSV2cat into the EcoRV site of Bluescript KS+. Ends were blunt. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Tsonis PA, et al. CAT constructs with convenient sites for cloning and generating deletions. Nucleic Acids Res. 16: 7745, 1988. PubMed: 3166134 Panagiotis A Tsonis, personal communication |
| Shipped： | frozen |