| 产品名称: | Human genomic DNA (PZ-HPV-7) |
|---|---|
| 商品货号: | TS191621 |
| Product Format: | frozen Approx. 10 µg |
| Storage Conditions: | -20°C or colder in a manual defrost freezer. Avoid freeze-thawing. |
| Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Introduction: | Cell Line Description: Epithelial cells cultured from normal tissue of the peripheral zone of the prostate from a 70-year-old Caucasian male were transfected with HPV-18 DNA. Incorporation of HPV-18 DNA was confirmed by polymerase chain reaction; specific amplification of a 160-base pair fragment of the HPV-18 E6-transforming region was noted. Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 oncoprotein of HPV. The cells are negative for prostate specific antigen (PSA). See www.atcc.org for more information |
| Quality Control Specifications: | DNA Quality Control Information: ·xa0xa0xa0xa0xa0xa0xa0xa0 > 100 kb in size by agarose gel electrophoresis ·xa0xa0xa0xa0xa0xa0xa0xa0 No RNA detected in the agarose gel ·xa0xa0xa0xa0xa0xa0xa0xa0 A260/A280 ratio ³ 1.8 ·xa0xa0xa0xa0xa0xa0xa0xa0 Tested and verified for PCR amplification and restriction digestion. |