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微信小章| 产品名称： | L1.84 |
|---|---|
| 商品货号： | TS191673 |
| Designations： | L1.84 |
| Species： | Homo sapiens, human |
| Depositors： | P Devilee |
| Vector： | Construct size (kb): 4.68 |
| Insert： | DNA: genomic Insert lengths(kb): 0.680 Tissue: placenta Gene product: DNA Segment, repetitive D18Z1 |
| Insert Size (kb)： | 0.680 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.68, 0.68; HindIII--4.3; BamHI--4.3; PstI--3.5, 0.7; PvuII--uncut. This represents a variant of alphoid DNA repeat with an estimated copy number of approximately 2000 per haploid genome. With less stringent washing conditions, the probe detects some signal on chromosomes 2, 8, 9, and 20 in addition to the strong signal on 18. EcoRI multimers of 4, 6, 8, 10, and 12 segregate with chromosome 18 in mapping panels. |
| References： | Devilee P, et al. Two subsets of human alphoid repetitive DNA show distinct preferential localization in the pericentric regions of chromosomes 13, 18, 21. Cytogenet. Cell Genet. 41: 193-201, 1986. PubMed: 3011362 Devilee P, et al. Sequence heterogeneity within the human alphoid repetitive DNA family. Nucleic Acids Res. 14: 2059-2073, 1986. PubMed: 3960717 Choo KH, et al. A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res. 19: 1179-1182, 1991. PubMed: 2030938 Alexandrov IA, et al. Chromosome-specific alpha satellites: two distince families on human chromosome 18. Genomics 11: 15-23, 1991. PubMed: 1765373 |