| 产品名称: | pTSEC |
|---|---|
| 商品货号: | TS191689 |
| Designations: | pTSEC |
| Depositors: | EW Miles |
| Biosafety Level: | 1 |
| Vector Information: | Size (kb): 5.6799998283386230 Vector: pTSEC (plasmid) Promoters: Promoter Ptrc Construction: pSTR4-2 Marker(s):ampR Construct size (kb): 5.679999828338623 Features: primer site: 5-TTGACAATTAATCATCCGGC-3 promoter: Ptrc terminator: rrnBT1 terminator: rrnBT2 |
| Applications: | cloning vector |
| Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.6; PstI--5.6; EcoRI/PstI--4.4, 1.3. Overproduces yeast cystathionine beta-synthase (truncated). |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions: | Temperature: 37.0°C |
| References: | Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046 |
| Related Products: | component of:ATCC 87809 |
| Shipped: | frozen |
| Shipping Information: | xa0Frozen glycerol stock of E. coli containing the plasmid. |