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| 产品名称： | pGBM7 |
|---|---|
| 商品货号： | TS191698 |
| Designations： | pGBM7 |
| Depositors： | J Geiselmann |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 7.646
Vector: pGBM7 (plasmid)
Construction: pGBM3
Marker(s): spcR,strR
Construct size (kb): 7.646
Features: marker(s): strR/spcR replicon: pSC101 MCS: XhoI...StuI coding sequence: luxC coding sequence: luxD coding sequence: luxE |
| Applications： | pGBM7 expresses constitutively the luxCDE genes, providing the cell with saturating concentrations of the aldehyde substrate necessary for the reaction of bacterial luciferases. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.6; ClaI--6.3, 1.3; BglI--6.5, 1.1. When this plasmid is introduced into a bacterial cell the activity of a test promoter can be measured using the small luxAB cassette (2.1 kb) as a reporter gene. pGBM7 expresses constitutively the luxCDE genes, providing the cell with saturating concentrations of the aldehyde substrate necessary for the reaction of bacterial luciferases. pGBM7 was constructed by inserting PCR amplified luxC and luxDE, including their ribosome binding sites, into pGBM3 (ATCC 87499) between the SalI and EcoRI sites. |
| Media： | ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin |
| Growth Conditions： | Temperature: 37°C |
| References： | Manen D, et al. A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322. Gene 186: 197-200, 1997. PubMed: 9074496 |
| Shipped： | freeze-dried |