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微信小章| 产品名称： | pHP1 plasmid in E. coli GC10 |
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| 商品货号： | TS191723 |
| Designations： | pHP1 plasmid in E. coli GC10 |
| Species： | Homo sapiens, human |
| Depositors： | Roel Nusse |
| Applications： | The product of this reaction was then PCR amplified again using another forward primer outside the protein encoding domain and a degenerate set of reverse primers to a highly conserved stretch FHWCC. |
| Vector： | Construct size (kb): 3.95
Insert site: SmaI |
| Insert： | DNA: cDNA
Gene: Wingless-type MMTV integration site family member 3, WNT3
Insert size (kb): 1.0 |
| Putative ID： | wingless-related MMTV integration site 3 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | xa0Frozen glycerol stock of Escherichia coli GC10 containing the plasmid. |
| Comments： | This is a clone of the human WNT3 in the pBluescript M13 vector.
The insert was the result of a PCR amplification of mRNA from fetal tissue using a forward primer derived from the first exon of human WNT3 outside the coding domain and a set of degenerate reverse primers to the reverse complement of the mouse Wnt-3 gene corresponding to the last 4 amino acids and the stop codon. The product of this reaction was then PCR amplified again using another forward primer outside the protein encoding domain and a degenerate set of reverse primers to a highly conserved stretch FHWCC. Restriction digests of the clone gave the following results (in kb): NotI - 4; NotI/EcoRI - 3.0, 1.0; HindIII/XhoI - 4.0; SmaI - uncut; NotI/HindIII - 3.0, 1.0; HindIII - 4.0. - ATCC staff Due to the use of a gene internal 3 primer in the PCR reaction, the depositorxa0did not retrieve the complete protein encoding sequence of human WNT3. Ref |
| References： | Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993. |
Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.