微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pMCT96.1 RW-110 |
|---|---|
| 商品货号： | TS191736 |
| Designations： | pMCT96.1 RW-110 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 4.5 Gene product: DNA Segment, single copy D9S14 Alleles: R5, R1, R2, R6, R3, R4 |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): AccI--4.6, 1.95; BamHI--2.75, 2.25, 1.35; EcoRI--3.5, 3.3; HindIII--5.1, 1.7; PstI--3.2, 1.2, 0.81, 0.56, 0.36. 0.32. Bands detected after a MspI digest are faint. RsaI digest demonstrates the polymorphism best. The probe should be hybridized to total human DNA (50-100 ug/ml) for 2 h before incubation with the filter (42C) because the probe contains repetitive DNA. Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pMCT96.1 and pMCT96.2) on chromosome 9q D9S14. Nucleic Acids Res. 16: 9881, 1988. PubMed: 2903483 Peter J OConnell, personal communication |