微信小陈
微信小章
产品简介
购买须知
| 产品名称： | c131 cosmid131 |
|---|---|
| 商品货号： | TS191831 |
| Designations： | c131 cosmid131 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 35.0 |
| Insert： | DNA: genomic Insert lengths(kb): 35.0 Gene product: DNA Segment, single copy D17S78 Alleles: A1, B1, B2, A2 |
| Insert Size (kb)： | 35.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--14.5, 12, 11, 1.6, 1.5; BamHI--22, 12.8, 6.5, 2.6, 1.7, 1.4, 0.6; SalI--26, 10; EcoRI--22, 16.5, 6.8, 2.9, 2.6, 2.2; XhoI--27, 9. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. MspI RFLP detected with a 6.5 kb BamHI fragment. Of 41 individuals, 30 were heterozygous for one or both RFLPs detected by cosmid 131 (ATCC 59310, 59311, 59312, 59313). Enzyme(s) not detecting polymorphism: TaqI, RsaI, EcoRI, HindIII, PvuII, BamHI, SacI, XbaI, DraI, BstEII. |
| References： | Luty J, et al. RFLPs revealed by cosmid 131 HGM9 No. D17S78. Nucleic Acids Res. 16: 6250, 1988. PubMed: 2899873 |