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| 产品名称： | Tetrahymena rostrata (Kahl) Corliss |
|---|---|
| 商品货号： | TS191915 |
| Strain Designations： | TRO1 |
| Application： | Morphological and molecular characterization |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Renal organ from a garden snail (Helix aspersa), heliciculture farm, Valga, Spain |
| Product Format： | test tube |
| Storage Conditions： | Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage Freeze-dried Cultures: 2-8°C Live Cultures: See Protocols section for handling information |
| Type Strain： | no |
| Comments： | Morphological and molecular characterization |
| Medium： | ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X) ATCC® Medium 357: Tetrahymena medium ATCC® Medium 383: Haskins agar for Tetrahymena |
| Growth Conditions： | Temperature: 18°C to 25°C
Culture System: Axenic |
| Cryopreservation： | Reagents RM-9 Media for cryopreservation of Tetrahymena Proteose Peptone (Difco 0120), 5.0 g Tryptone, 5.0 g K2HPO4, 0.2 g Glucose, 1.0 g Liver extract, 0.1 g Glass distilled water, 1.0 L Dissolve components in glass distilled H2O and autoclave. Dryl’s Salt Solution 0.1 M NaH2PO4 . 3H20, 10.0 mL 0.1 M Na2HPO4 . 7H20, 10.0 mL 0.1 M Sodium citrate . 2H20, 15.0 mL 0.1 M CaCl2 . 2H20 , 15.0 mL Distilled water , 950.0 mL Add the first 3 components to the distilled H2O and mix thoroughly. Add the CaCl2 solution and mix thoroughly. (Adding the solutions in the order indicated will avoid the precipitation of Ca salts.) Harvest and Preservation
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| Name of Depositor： | R Iglesias |
| References： | Segade P, et al. Morphological and molecular characterization of renal ciliates infecting farmed snails in Spain. Parasitology 136: 771-782, 2009. PubMed: 19402940 |