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| 产品名称： | p58-CL6 |
|---|---|
| 商品货号： | TS191985 |
| Designations： | p58-CL6 |
| GenBank Number： | U24074 |
| Species： | Homo sapiens, human |
| Depositors： | N Wagtmann |
| Vector： | Construct size (kb): 5.71999979019165 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSPORT1 Intact vector size: 4.109 Type of vector: plasmid Vector end: SalI Vector end: NotI Cloning sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI Sse8387I PstI Polylinker sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI Sse8387I PstI Host range: Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: SP6, ->, 164-180 MCS: AatII...PstI, ->, 191-285 promoter for in vitro transcription: T7, insert detection: lacZ, repressor gene: lacI, replicon: pMB1, 1903-1903 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: KIR Other gene symbols: NKB1 Gene name: killer cell inhibitory receptor ORFs seq. position: GDB probe: GDB:698358 () Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 q13.4; 19, 374.860000 cR Tissue: NK3.3 cell line Type of DNA: cDNA Insert 5 end: Modification: SalI linker Insert 3 end: Modification: NotI linker Insert size (kb): 1.61 Cross references: DNA Seq. Acc.: U24074 Nucleotides 1-1579 of the insert correspond to nucleotides 1-1579 of U24074. Insert lengths(kb): 1.610000014305115 Tissue: NK3.3 cell line Gene product: killer cell inhibitory receptor KIR |
| Insert Size (kb)： | 1.610 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4, 1.6; EcoRV--4.5, 1.5; PstI--5.0, 0.4, 0.35, 0.1; PvuII--4.0, 1.4, 0.6; XhoI--6.0. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--1.01; NdeI--0.93; PstI--0.07, 0.46, 0.54, 0.87; PvuII--1.15; SacI--0.48; StuI--1.08; XhoI--0.65. Encodes a variant recognized by monoclonal antibody GL183 but not EB6. |
| References： | Wagtmann N, et al. Molecular clones of the p58NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity 2: 439-449, 1995. PubMed: 7749980 |