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| 产品名称： | pUCD4 plasmid in E. coli |
|---|---|
| 商品货号： | TS191999 |
| Designations： | pUCD4 plasmid in E. coli |
| Depositors： | CI Kado |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 13.0 Vector: pUCD4 (plasmid) Promoters: Promoter none Construction: pUCD2 (ATCC 37342) Marker(s):ampR,kanR,spcR,tetR Construct size (kb): 13.0 Features: marker(s): ampR, kanR, spcR, tetR promoter: none replicon: pMB1, pSa151 terminator: none enhancer: none |
| Applications： | shuttle vector |
| Comments： | A broad host range amplifiable cloning vector for use in complementation studies of cloned inserts in Agrobacterium tumefaciens. The unique PvuII site of pUCD2 (ATCC 37342) was changed to an XbaI site (using linkers) to create pUCD4. This vector is the same as pUCD2 except for the XbaI restriction site.The pUCD2 vector was constructed by fusing pBR322 with the pSAl51 plasmid and was shown to have lost 2.5 kb on each side of the BglII site giving it a positive vir region complementation in Agrobacterium tumefaciens. Restriction digests of the clone give the following sizes (kb): PstI--13; EcoRV--13; KpnI--13; SalI--13. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Close TJ, et al. Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58. Plasmid 12: 111-118, 1984. PubMed: 6095350 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the cosmid |