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| 产品名称： | pUFR034 |
|---|---|
| 商品货号： | TS192034 |
| Designations： | pUFR034 |
| Depositors： | DW Gabriel |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.6999998092651370 Vector: pUFR034 (cosmid) Promoters: Promoter lac Construction: pUC18, pSa747, pHC79, pUCD550, pUC4Kan, pLAFR3 Marker(s):neoR Construct size (kb): 8.699999809265137 Features: insert detection: lacZ marker(s): neoR promoter: lac replicon: oriW |
| Applications： | shuttle vector vector for constructing genomic libraries vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--8.7; PstI--6.4, 1.35, 1.2; SmaI--7.0, 1.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Uses interruption of lacZalpha. Shuttle vector permitting visual detection of recombinants. The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture. The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - cos (pHC79) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+). |
| Media： | ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | DeFeyter R, et al. Small, stable shuttle vectors for use in Xanthomonas. Gene 88: 65-72, 1990. PubMed: 2341039 |
| Shipped： | freeze-dried |