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微信小章| 产品名称： | 465.20 |
|---|---|
| 商品货号： | TS192125 |
| Designations： | 465.20 |
| GenBank Number： | JO3236 |
| Species： | Mus musculus, mouse |
| Depositors： | D Nathans |
| Vector： | Construct size (kb): 4.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.91700005531311 Tissue: activated 3T3 cell line Gene product: Jun-B oncogene(jun B proto-oncogene) Junb |
| Insert Size (kb)： | 1.917 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following unique sites (in bp from the T7 promoter side 5): HindIII--6; SmaI--269; FokI--318; AccI--851; SacI--925; DraI--1025; StuI--1451; XhoI--1513; EcoRI--1576. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.5, 0.3; HindIII--4.6; BamHI--4.2, 0.4; PstI--4.6; SacI--3.8, 0.8; SmaI--4.4, 0.3. Contains the complete coding sequence, 487 bp 3 untranslated sequence, and about 100 nt poly(A). Under moderately stringent conditions (0.5 M NaCl, 65C), the probe detects a genomic EcoRI fragment larger than 12 kb in humans, a 9 kb fragment in chickens, an 8 kb fragment in mouse and other less prominent bands. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Ryder K, et al. A gene activated by growth factors is related to the oncogene v-jun. Proc. Natl. Acad. Sci. USA 85: 1487-1491, 1988. PubMed: 3422745 Lau LF, Nathans D. Expression of a set of growth-rleated immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc. Proc. Natl. Acad. Sci. USA 84: 1182-1186, 1987. PubMed: 3469660 Howard TA, et al. A linkage map of mouse chromosome 8: further definition of homologous linkage relationships between mouse chromosome 8 and human chromosomes 8, 16, and 19. Genomics 10: 207-213, 1991. PubMed: 1675193 Daniel Nathans, personal communication |