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| 产品名称： | Sandfly fever sicilian virus |
|---|---|
| 商品货号： | TS192130 |
| Classification： | Bunyaviridae, Phlebovirus |
| Deposited As： | Phlebotomus virus (Sicily) |
| Agent： | Sandfly fever sicilian virus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | BHK-21 C-13 (ATCC® CCL-10™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection:xa0Plate cells 24-48 hours prior to infection and infect when cultures are 30-50% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium Incubation: 4-5 days |
| Name of Depositor： | SE Grossberg, Medical College of Wisconsin |
| Chain of Custody： | ATCC |