| 产品名称: | c9205 9205 |
|---|---|
| 商品货号: | TS192165 |
| Designations: | c9205 9205 |
| GenBank Number: | U00060 U10556 |
| Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors: | MV Olson, L Riles |
| Vector: | Construct size (kb): 48.20000076293945 |
| Insert: | DNA: genomic Insert lengths(kb): 41.76200103759766 Gene product: protein kinase GAR1 |
| Insert Size (kb): | 41.762 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products: | component of:ATCC 77284 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 5.764, 2.526, 1.951, 2.143, 2.042, 0.679, 0.621, 3.448, 1.163, 0.938, 0.570, 0.485, 0.947, 1.676, 0.885, 6.831, 2.565, 3.500, 1.567, 1.224. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.57, 0.63, 0.64, 0.77, 0.85, 0.92, 1.0, 1.2, 1.4, 1.6, 1.7, 2.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70252, 70715, 70755. Corresponds to nt 245038-286800 of the sequence of chromosome VIII. |
| Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References: | Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |