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| 产品名称： | pE194-cop6 BSGC 1E7 |
|---|---|
| 商品货号： | TS192377 |
| Designations： | pE194-cop6 BSGC 1E7 |
| Depositors： | CPC International, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Bacillus subtilis BSV IE7 |
| Vector Information： | Construct size (kb): 3.599999904632568 |
| Applications： | shuttle vector |
| Comments： | For full induction, the plasmid requires a period of induction by subinhibitory concentrations of erythromycin. Temperature sensitive. Plasmid is a high copy mutant of pE194, a natural isolate from Staphylococcus aureus that confers resistance to macrolide, lincosamide and streptogramin type B (MLS) antibiotics. |
| Media： | ATCC® Medium 18: Trypticase Soy Agar/Broth |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Dean DH, Dooley MM. Plasmid cloning vector for Bacillus subtilis. US Patent 4,419,450 dated Dec 6 1983 Weisblum B, et al. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643, 1979. PubMed: 104975 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |