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微信小章| 产品名称： | php53B pHp53 |
|---|---|
| 商品货号： | TS192390 |
| Designations： | php53B pHp53 |
| GenBank Number： | X02469 |
| Species： | Homo sapiens, human |
| Depositors： | D Givol |
| Vector： | Construct size (kb): 6.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: SV40-transformed fibroblast Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) TP53 Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 2.2; EcoRI--6.6; HindIII--6.6; PstI--5.3, 1.2; AvaI--4.9, 1.6. The insert begins 135 bp upstream of the first ATG. It includes the entire coding sequence (for 393 amino acids) and 3 sequences. The Alu sequence in php53C-1 has been removed. The BanII and ScaI polymorphisms demonstrated codominant segregation in two families (12 individuals). Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, PstI, PvuII, RsaI, TaqI, XbaI. |
| References： | Zakut-Houri R, et al. Human P53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4: 1251-1255, 1985. PubMed: 4006916 Masharani U, et al. BanII and ScaI RFLPs at the human p53 gene locus. Nucleic Acids Res. 16: 7757, 1988. PubMed: 2901071 de la Calle-Martin O, et al. MspI polymorphism of the human p53 gene. Nucleic Acids Res. 18: 4963, 1990. PubMed: 1697680 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 |