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| 产品名称： | pdeltablue |
|---|---|
| 商品货号： | TS192400 |
| Designations： | pdeltablue |
| Depositors： | ME Brandt |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5069999694824220 Vector: pdeltablue (plasmid) Promoters: Promoter lambda PL Construction: pfXblue Marker(s):ampR Construct size (kb): 3.506999969482422 Features: insert detection: lacZ marker(s): ampR promoter: lambda PL replicon: pMB1 |
| Applications： | expression vector vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45. Restriction digests of the plasmid give the following sizes (kb): BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063, 1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25. Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3 end of the insert. The vector supplies the AUG initiator codon. The insert to be cloned should have a phosphorylated 5 blunt end with the first base corresponding to the first base of the first codon. Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ allows blue/white screening of colonies. Constructed from pfXblue (ATCC 77333) by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943). The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brandt ME, Vickery LE. Expression and characterization of human mitochondrial ferredoxin reductase in Escherichia coli. Arch. Biochem. Biophys. 294: 735-740, 1992. PubMed: 1567230 Mark E Brandt, personal communication |
| Shipped： | freeze-dried |