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| 产品名称： | Escherichia coli 1046 (pCRE1) |
|---|---|
| 商品货号： | TS192413 |
| Designations： | Escherichia coli 1046 (pCRE1) |
| Depositors： | IN Maruyama |
| Applications： | Produces protein recombinase, site-specific |
| Vector： | Construct size (kb): 5.960
Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: ScaI Vector end: ScaI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMIxa0BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaIxa0PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert end: PvuII Insert end: PvuII Insert size (kb): 1.6 Cross references: Gene product: recombinase, site-specific cre
Target Gene: recombinase, site-specific |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Related Products： | Component of: ATCC 77369 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze-dried E. coli 1046 |
| Comments： | This E. coli contains the pCRE1 plasmid and expresses the Cre recombinase from a lacZ promoter.xa0 Insert-containing lambdaMGU2 (77367) cannot grow productively in this RecA- strain, so excised phagemid are recovered as ampR surviviors of phage infection. Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.05; XmnI--3.1, 2.4, 0.45; HindIII--5.2, 0.75; BamHI--4.4, 1.55; EcoRI/PstI--3.5, 1.4, 0.7, 0.35. |
| References： | Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972 |