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| 产品名称： | pAd.CB-CFTR purified plasmid |
|---|---|
| 商品货号： | TS192482 |
| Designations： | pAd.CB-CFTR purified plasmid |
| Species： | Homo sapiens, human |
| Depositors： | University of Michigan Medical Center, JM Wilson, University of Michigan Medical Center |
| Vector： | Vector: pAd.CMV.Link.1 Vector type: plasmid Vector size (kb): 6.668 Cloning sites: EcoRV ClaI HindIII KpnI SalI XbaI XhoI SpeI Markers: ampR Features (with orientation and position (nt) when available): Restriction site: EcoRI, -->, 2 Other: Ad mu 0-1,xa0 Promoter: CMV immediate early enhancer and B-actin promoter, -->, 381-903 Restriction site: NcoI, 681 Restriction site: XbaI, 979 Restriction site: XhoI, 995 Other: Intron, --> Restriction site: NotI, 1174 MCS: EcoRV…SpeI,xa0--> 1181-1253 Restriction site: NotI, -->, 1834 Enhancer: SV40 PolyA, -->, 1269-1466 Restriction site: XbaI, SalI, 1473-1479 Restriction site: NcoI SmaI, 1834-2287 Other: Ad 9-16 mu, --> Restiction site: NcoI, 3619 Replicon: pAT153 |
| Insert： | Insert size (kb): 6.7 kb (see above for more explanation) Gene: cystic fibrosis transmembrane conductance regulator CFTR DNA: cDNA Insert ends: XbaI |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | 200 ng/vial in ~5 µL TE buffer |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.2, 2.1, 1.6, 1.1; HindIII--10.0, 0.9; BamHI--6.0, 2.5, 1.6, 0.6, 0.2; BglII--10.6, 0.4; PstI--5.1, 2.3, 1.2, 0.8, 0.7, 0.5, 0.4. Contains the full-length cDNA with expression regulated in mammalian cells by a CMV enhancer and a beta-actin promoter. Digestion of the construct with XbaI yields 2 major fragments of 4.3 kb, containing most of the CFTR sequence and the polyadenylation signals, and 6.7 kb, containing the adenovirus and plasmid vector sequences. Distributed in aliquots of 200 ng (40 ng/ul). The order of the major features in this plasmid is: NheI - Ad 0-1 - CMV enhancer - beta-actin promoter - XbaI - insert (contains 1 XbaI site) - SV40 polyadenylation signal - XbaI - Ad 9.2-16.1 - pAT153. |
| References： | James M Wilson, personal communication Human Gene Therapy 5: 501-519, 1994. EP19940916720 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |