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微信小章| 产品名称： | pAQP1 UTR-01749, WI-9113, pCHIP |
|---|---|
| 商品货号： | TS192483 |
| Designations： | pAQP1 UTR-01749, WI-9113, pCHIP |
| GenBank Number： | M77829 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.760000228881836 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS- Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, ->, 3-459 primer site: M13-20, -> promoter for in vitro transcription: T7, -> insert detection: lacZ, MCS: SacI...KpnI, ->, 657-759 promoter for in vitro transcription: T3, primer site: reverse, replicon: pMB1, 1032-1972 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AQP1 Other gene symbols: CHIP28 Gene name: aquaporin 1 (channel-forming integral protein, 28 kD) Contains complete coding sequence?: N Chromosome: 7; Localization: 7 p14; 7, 84.8799999 cR Tissue: bone marrow Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 2.8 Cross references: DNA Seq. Acc.: M77829 Nucleotides 1-1283 of the insert correspond to nucleotides 53-1335 of M77829. Insert lengths(kb): 2.799999952316284 Tissue: bone marrow Gene product: aquaporin 1 (channel-forming integral protein, 28 kD) AQP1 |
| Insert Size (kb)： | 2.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.8; HindIII--4.4, 1.5; SmaI--5.0, 0.8; KpnI--5.8; BglII--5.8. The insert contains the following restriction sites (approximate kb from the 5 end): SmaI--0.80; HindIII--1.28. The insert begins at nt +15 relative to the initiation ATG, and contains about 1.3 kb at the 3 end that has not been sequenced. |
| References： | Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319 Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524 Preston GM, et al. The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel. J. Biol. Chem. 268: 17-20, 1993. PubMed: 7677994 Bondy C, et al. Developmental gene expression and tissue distribution of the CHIP28 water-channel protein. Proc. Natl. Acad. Sci. USA 90: 4500-4504, 1993. PubMed: 8506291 Moon C, et al. The human aquaporin-CHIP gene. J. Biol. Chem. 268: 15772-15778, 1993. PubMed: 8340403 Preston GM, et al. Membrane topology of aquaporin CHIP. J. Biol. Chem. 269: 1668-1673, 1994. PubMed: 7507481 Jung JS, et al. Molecular structure of the water channel through aquaporin CHIP. J. Biol. Chem. 269: 14648-14654, 1994. PubMed: 7514176 |