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| 产品名称： | p5-1-30 |
|---|---|
| 商品货号： | TS192512 |
| Designations： | p5-1-30 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Applications： | The most efficient detection of RFLPs at the D2S3 locus is by use of the cosmid 1-5 subclones p5-1-25 (ATCC 59684) and p5-2-96 (ATCC 59000). |
| Vector： | Construct size (kb): 6.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.399999976158142 Gene product: DNA Segment, single copy D2S3 Alleles: C4, J10, A1, B1, B2, C1, C2, C3, D2, G4, J1, J3, J4, J5, J6, J7, J8, K2, A2, D1, E1, E2, G1, G2, G3, J2, J9, K1 |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Constructed by inserting Sau3A fragment into BamHI site. Insert contains KpnI sites. Restriction digests of the clone give the following sizes (kb): KpnI--6.0, 0.62; EcoRI--5.3, 1.4; HindIII--6.7; SalI--6.7; BamHI--6.7. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert was derived from cosmid 1-5. The most efficient detection of RFLPs at the D2S3 locus is by use of the cosmid 1-5 subclones p5-1-25 (ATCC 59684) and p5-2-96 (ATCC 59000). Use of p5-1-30 (TS192512) and p5-1-32 (ATCC 59002) probably adds little to the PIC. |
| References： | Litt M, White RL. A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82: 6206-6210, 1985. PubMed: 2994065 Demenais F, et al. Robustness and power of the unified model in the analysis of quantitative measurements. Am. J. Hum. Genet. 38: 228-234, 1986. PubMed: 3946424 |