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| 产品名称： | CrmA T291R |
|---|---|
| 商品货号： | TS192597 |
| Designations： | CrmA T291R |
| Species： | Cowpox virus |
| Depositors： | G Salvesen |
| Applications： | produces protein cytokine response modifier A, inactive mutant |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pFlag-1 deriv Intact vector size: Type of vector: phagemid Vector end: NcoI Vector end: SalI Cloning sites: HindIII EcoRI XbaI SalI BglII XhoI Polylinker sites: HindIII EcoRI XbaI SalI BglII XhoI Construction: pFLAG, FLAG/ompA sequences deleted Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: tac replicon: pMB1, f1 repressor gene: lacI transcription terminator: rrnB Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Cowpox Gene symbol: CrmA Gene name: cytokine response modifier A Contains complete coding sequence?: Y Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.2 Cross references: Insert lengths(kb): 1.200000047683716 Gene product: cytokine response modifier A CrmA Target Gene: cytokine response modifier A, inactive mutant |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NcoI/SalI--6.1, 0.85; PstI--4.2, 1.8, 0.9; BamHI--6.5, 0.45. Insert encodes an inactive control protein tagged with hexahistidine at the N-terminus. The T291R mutation occurs within the reactive site loop. |
| Classification： | Poxviridae, Orthopoxvirus |
| References： | Tewari M, et al. Yama/CPP32beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801-809, 1995. PubMed: 7774019 |