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| 产品名称： | Fcwf-4 Fcwf |
|---|---|
| 商品货号： | TS192645 |
| Organism： | Felis catus, cat |
| Tissue： | fetus, whole |
| Cell Type： | macrophage |
| Product Format： | frozen |
| Morphology： | spindle to stellate |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | peritonitis |
| Age： | fetus |
| Applications： | It is used to propagate tissue culture adapted Feline coronavirus (Feline infectious peritonitis virus, FIPV).
|
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This continuous feline cell line was developed in 1979.xa0 |
| Virus Susceptibility： | Feline infectious peritonitis virus
|
| Comments： | The cells possess some characteristics of macrophages (nonspecific esterase, phagocytic activity, Fc receptors). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Interval: Maintain cultures at a cell concentration between 2 x 104xa0and 1 x 105xa0cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature:xa037°C |
| Population Doubling Time： | 31 hrs |
| Name of Depositor： | NC Pedersen |
| Year of Origin： | 1979 |
| References： | Jacobse-Geels HE, Horzinek MC. Expression of feline infectious peritonitis coronavirus antigens on the surface of feline macrophage-like cells. J. Gen. Virol. 64: 1859-2866, 1983. PubMed: 6886678 Pedersen NC, et al. Infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture. Am. J. Vet. Res. 42: 363-367, 1981. PubMed: 6267959 |