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| 产品名称： | cYN5.64 |
|---|---|
| 商品货号： | TS192660 |
| Designations： | cYN5.64 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation observed in 60 families. |
| Vector： | Construct size (kb): 48.0 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy D5S82 Alleles: B1, B2, A1, A2 |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--8.6, 6.6, 4.8, 3.0, 2.8, 2.7, 2.15, 1.7, 1.5, 1.25, 1.2, 0.8; BamHI--20.0, 13.0, 8.6, 5.0; PstI--8.4, 5.65, 5.05, 3.8, 3.55, 3.4, 2.8, 2.4, 1.8, 1.65, 1.4, 0.85; HindIII--8.0, 6.8, 6.0, 5.7, 3.6, 3.4, 2.0 (doublet), 1.1. Codominant segregation observed in 60 families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: RsaI, MspI. |
| References： | Raymond L White, personal communication |