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微信小章| 产品名称： | LL04NS01 |
|---|---|
| 商品货号： | TS192696 |
| Designations： | LL04NS01 |
| Species： | Homo sapiens, human |
| Depositors： | M Van Dilla, JA Garnes |
| Origin： | hamster (CHO)/human lymphocyte UV20HL21-27 hybrid |
| Vector： | Tissue: hamster (CHO)/human lymphocyte UV20HL21-27 hybrid Chromosome: 4 DNA: genomic Digest: Complete Insert size range: 2.0 kb average Genome Equivalent: 0.800000011920929 Coverage: Clones for "complete" coverage: 31000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen (volume: 0.2 ml) |
| Comments： | The #4 peak was well-isolated from hamster background with little debris. According to fluorescence in situ hybridization using genomic hamster DNA with sorted chromosomes, there is 17% hamster DNA. About 83% of the library is human (and 17% hamster) by plaque hybridization. Of 2315 randomly selected plaques screened: 224 human single copy greater than 600 bp; 1106-human probably repetitive; 595-inserts absent or less than 600 bp; 62-hamster single copy greater than 600 bp; 328-hamster probably repetitive. There is approximately 20% hamster impurity. Of 47 unique sequence clones tested, 47 map to chromosome 4. The average insert size is 2.0 kb. Of the 224 single copy inserts screened, all detected an appropriate HindIII fragment on chromosome 4; some detected additional fragments not on 4; some also cross-hybridized to hamster sequences. The library LL04NS01, TS192696, 0.8 genomic equivalents, is distributed together with LL04NS02, ATCC 57745, 10.0 genomic equivalents, because there is a possibility that a sequence in one may not be found in the other. |
| References： | . Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986. Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319 Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 Gilliam TC, et al. Isolation of polymorphic DNA fragments from human chromosome 4. Nucleic Acids Res. 15: 1445-1458, 1987. PubMed: 2881276 Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744 Marvin Van Dilla, personal communication |