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| 产品名称： | pCMV-drosE2F1 |
|---|---|
| 商品货号： | TS192810 |
| Designations： | pCMV-drosE2F1 |
| GenBank Number： | U10184 |
| Species： | Drosophila melanogaster, fruit fly |
| Depositors： | JR Nevins |
| Applications： | in another host, produces protein E2F transcription factor 1 |
| Vector： | Construct size (kb): 9.100000381469727 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.400000095367432 Tissue: imaginal discs Gene product: E2F transcription factor 1 E2f1 Target Gene: E2F transcription factor 1 |
| Insert Size (kb)： | 4.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 4.5 (doublet); EcoRV--7.1, 1.9; HindIII--6.6, 2.4. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--1.36; BglII--1.78; EcoRV--1.15, 3.00; HindIII--1.90; PstI--1.09, 1.51, 2.75, 2.95; PvuI--2.73, 3.08; PvuII--1.31, 2.35, 2.72; SalI--0.93, 1.40; XhoI--0.46, 1.44. |
| References： | Ohtani K, Nevins JR. Functional properties of a Drosophila homolog of the E2F1 gene. Mol. Cell. Biol. 14: 1603-1612, 1994. PubMed: 8114698 |