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购买须知
| 产品名称： | mWAP |
|---|---|
| 商品货号： | TS192863 |
| Designations： | mWAP |
| Species： | Mus musculus, mouse |
| Depositors： | LG Hennighausen |
| Vector： | Construct size (kb): 12.0 |
| Insert： | DNA: genomic Insert lengths(kb): 7.199999809265137 Tissue: liver Gene product: whey acidic protein Wap |
| Insert Size (kb)： | 7.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | SphI, KpnI, XbaI, XhoI, SalI and BamHI sites are probably unique within the insert. Insert contains the following sites separated by (kb) (approximate): EcoRI- 0.58- BglII- 0.2- BglII- 0.2- SphI- 1.1- XhoI- 0.43- KpnI- 1.5- PstI- 0.22- SalI- 1.26- BamHI- 1.53- EcoRI. Approximate sizes of fragments (kb)(from depositor): EcoRI--7.0, 3.2; EcoRI+KpnI--4.4, 3.2, 2.6; EcoRI+BamHI--5.5, 3.2, 1.5; EcoRI+SalI--4.2, 3.2, 2.8; EcoRI+XhoI--4.9, 3.2, 2.1; EcoRI+XbaI--4.5, 3.2, 2.5. Restriction digests of the clone give the following sizes (kb): EcoRI--8.4, 3.6; BamHI--12.2; SalI--12.2; EcoRI/BamHI--6.4, 3.6, 1.8; EcoRI/SalI--5.0, 3.6, 3.2. The KpnI site is located in the first exon just 5 of the first AUG (position +24). More homology between mouse and rat WAP genes in 3 noncoding (91%) than coding (82%) regions. For 5 region, homology between mouse and rat genes is 83% and extends at least to position -325. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Campbell SM, et al. Comparison of the whey acidic protein genes of the rat and mouse. Nucleic Acids Res. 12: 8685-8697, 1984. PubMed: 6095207 Lothar G Hennighausen, personal communication |