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| 产品名称： | pmut-1 |
|---|---|
| 商品货号： | TS192891 |
| Designations： | pmut-1 |
| GenBank Number： | K02292 |
| Species： | Mus musculus, mouse |
| Depositors： | T Yokota |
| Vector： | Construct size (kb): 5.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.9300000071525574 Tissue: T-lymphocyte LB2-1 cell line Gene product: interleukin 2 Il2 |
| Insert Size (kb)： | 0.930 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.0; HindIII--4.6, 0.8; PstI--4.2, 1.2; PvuII--3.2, 2.4. The 1.0 kb (approx) BamHI fragment includes the cDNA of 0.93 kb which contains the complete coding region plus 5 and 3 untranslated sequences. The BamHI fragment also includes, at the 5 end, at least 102 bp of the original pcD vector. The BamHI fragment of pcD-mut-1 was cloned into the BamHI site of pBR322. There is also pcD vector sequence at the 3 end. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Yokota T, et al. Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc. Natl. Acad. Sci. USA 82: 68-72, 1985. PubMed: 3918306 Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 |