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| 产品名称： | Chlamydophila psittaci (Lillie) Everett et al. |
|---|---|
| 商品货号： | TS192976 |
| Classification： | Chlamydiaceae |
| Deposited As： | Ornithosis |
| Agent： | Chlamydophila psittaci (Lillie) Everett et al. |
| Strain： | Ornithosis strain Texas Turkey strain Texas turkey |
| Applications： | Addition of cycloheximide recommended for passage in cell culture. |
| Biosafety Level： | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | turkey spleen, Texas |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Centrifugation (2000 X g for 1 hr.) and addition of cycloheximide recommended for passage in cell culture. *Requests for this agent must carry signed statement assuming all risks and responsibility for lab handling. Triturate fluid several times to disperse chlamydia. For cell culture, centrifuge inoculumn, adsorb for 1 hour and feed with HEPES buffered culture medium. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Cytopathic effects (inclusions) in tissue culture Death of embryo |
| Recommended Host： | McCoy cells (ATCC CRL-1696) chicken embryo, yolk sac; mouse; turkey; McCoy cells (ATCC CRL-1696) |
| Growth Conditions： | Incubation: 5 days (approximately) |
| Name of Depositor： | J Schachter |
| Source： | turkey spleen, Texas |
| References： | Meyer KF, Eddie B. Characteristics of a psittacosis viral agent isolated from a turkey. Proc. Soc. Exp. Biol. Med. 83: 99-101, 1953. PubMed: 13064188 |