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| 产品名称： | Quantitative Synthetic Eastern equine encephalitis virus RNA |
|---|---|
| 商品货号： | TS193027 |
| Agent： | Quantitative Synthetic Eastern equine encephalitis virus RNA |
| Applications： | ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen Specification range: 1 x 105 to 1 x 106 copies/µL 100 µL per vial with Biomatrica RNAstable |
| Storage Conditions： | -70°C or colder |
| Intended Use： | The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent. |
| Comments： | Manufactured under ISO 13485 guidance Preparation includes fragments from capsid, envelope (E1 and E2), and nonstructural genes NSP1, NSP3, and 3’UTR. The following primers and probe can be used with this nucleic acid preparation Ref   Lambert AG, et al. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J. Clin. Microbiol. 41(1): 379-385, 2003. PubMed: 12517876:Forward primer: ACACCGCACCCTGATTTTACA Reverse primer: CTTCCAAGTGACCTGGTCGTC Probe: FAM-TGCACCCGGACCATCCGACCT-BHQ |
| Special Collection： | RNA |
| References： | Lambert AG, et al. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J. Clin. Microbiol. 41(1): 379-385, 2003. PubMed: 12517876 Zink SD, et al. Quadraplex qRT-PCR assay for the simultaneous detection of Eastern equine encephalitis virus and West Nile virus. Diagn. Microbiol. Infect. Dis. 77(2): 123-132, 2013. PubMed: 23891222 |