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| 产品名称： | YCp631 in E. coli |
|---|---|
| 商品货号： | TS193112 |
| Designations： | YCp631 in E. coli |
| Depositors： | J Thorner |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.26 Vector: YCp631 (plasmid) Construction: deletion of 2 micron ori, URA3 from YEp24, LYS2, CEN3-ARI Marker(s):ampR,tetR,LYS2 Construct size (kb): 12.26 Features: marker(s): ampR, LYS2, tetR replicon: pMB1, ARS1 centromere: CEN3 |
| Applications： | YC-type (centromeric) shuttle vector shuttle vector |
| Comments： | A general purpose Saccharomyces cerevisiae/Escherichia coli shuttle vector containing centromeric DNA. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Barnes DA, Thorner J. Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene. Mol. Cell. Biol. 6: 2828-2838, 1986. PubMed: 3023949 |
| Shipped： | freeze-dried |