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| 产品名称： | pSON8-JUN |
|---|---|
| 商品货号： | TS193207 |
| Designations： | pSON8-JUN |
| GenBank Number： | J04111 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. Positive control for SOS Recruitment System. |
| Vector： | Construct size (kb): 9.694000244140625 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSON8 Intact vector size: 9.469 Type of vector: plasmid Vector end: NcoI Vector end: SalI Cloning sites: NcoI SmaI HpaI SalI EagI Polylinker sites: NcoI SmaI HpaI SalI EagI HindIII MunI EcoRI XhoI Other unique sites: ApaI SpeI NheI BglII KpnI BamHI PvuI XbaI AhaII Construction: pUC Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): URA3, -> promoter: ADH, -> MCS: NcoI...EagI, -> coding sequence: SOS (human Son of sevenless), -> terminator: ADH, -> replicon: pMB1 marker(s): ampR, replicon: 2 micron ori Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: JUN Gene name: c-jun oncogene Contains complete coding sequence?: N Chromosome: 1; Localization: 1p32-31 Type of DNA: cDNA Insert 5 end: NcoI Insert 3 end: SalI Insert size (kb): 0.257 Cross references: DNA Seq. Acc.: J04111 Insert lengths(kb): 0.2569999992847443 Gene product: c-jun oncogene JUN |
| Insert Size (kb)： | 0.257 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--9.7; HindIII--6.0, 3.7; BamHI--9.7; NcoI/SalI--9.5, 0.25. SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein. Positive control for SOS Recruitment System. The insert contains the leucine zipper motif. |
| References： | Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808 |