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| 产品名称： | pNASSbeta |
|---|---|
| 商品货号： | TS193290 |
| Designations： | pNASSbeta |
| Depositors： | GR Macgregor |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.6999998092651370 Vector: pNASSbeta (plasmid) Construction: pUC19, pC4AUG beta-gal, pL1 Marker(s):ampR Construct size (kb): 6.699999809265137 Features: insert detection: lacZ marker(s): ampR replicon: pMB1 terminator: SV40 late |
| Applications： | contains easily purifiable cassette(s) for construction promoter-cloning vector reporter construct vector for other uses vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; HindIII--6.7; EcoRI/HindIII--4.0, 2.7; NotI--3.5, 3.2; BamHI--3.65, 2.85, 0.2. Promoter-less reporter plasmid permitting visual detection of transcription/translation activity by histochemical staining. Promoters may be introduced upstream of the lacZ cassette at the unique EcoRI site. The lacZ gene may be excised by NotI and replaced with alternative sequences. The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. |
| Media： | ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524 |
| Shipped： | freeze-dried |