Construct size (kb): 4.75

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SNRPD*
Gene name: small nuclear ribonucleoprotein, SM-D (lupus autoantigen)
Contains complete coding sequence?: U
Chromosome: 15; Localization: 15, 10.19 cR
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: J04615; EST: EST06410
Nucleotides 1-304 of the insert correspond to
nucleotides 1326-1023 of J04615 with 99% identity.
DNA Seq. Acc.: T08518; EST: EST06410; Primer: M13 Reverse;
THC id: THC122881

Insert lengths(kb): 1.5

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: small nuclear ribonucleoprotein, SM-D (lupus autoantigen) SNRPD*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; HindIII/NotI--3.2, 1.08, 0.44.

Based on the insert size and the alignment with GenBank accession J04615 (1326 nt, CDS 466-1188), this clone appears to contain most if not all of the coding region and the 3 untranslated region.