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| 产品名称： | pSupexp |
|---|---|
| 商品货号： | TS193389 |
| Designations： | pSupexp |
| Depositors： | T Subramanian |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.5000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 5.500 Type of vector: plasmid Cloning sites: AflII SpeI SacII NruI ClaI NotI NdeI SnaBI HpaI KpnI Polylinker sites: AflII SpeI SacII NruI ClaI NotI NdeI SnaBI HpaI KpnI Construction: pBR322, pSL1190, pRcCMV Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: HIV-1 LTR marker(s): ampR, neoR, G418R promoter: HIV-1 LTR replicon: pMB1 terminator: SV40 Vector: pSupexp (plasmid) Promoters: Promoter HIV-1 LTR Construction: pBR322, pSL1190, pRcCMV Marker(s):G418R,ampR,neoR Construct size (kb): 5.5 Features: marker(s): ampR, neoR, G418R promoter: HIV-1 LTR replicon: pMB1 terminator: SV40 enhancer: HIV-1 LTR |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.5; NotI--5.5; KpnI--5.5. The AflII and SpeI cloning sites are recommended for the 5 end of foreign DNA to prevent potential translation effects of ATG sequences in the multiple cloning site. Shuttle expression vector permitting levels of protein production in human cells high enough for immunoprecipitation. Contains two mammalian transcription units: the foreign DNA regulated by HIV-1 LTR and an SV40 polyadenylation sequence, and HIV-1 tat and neo regulated by an SV40 early promoter and polyadenylation sequence. The order of the major features in this plasmid is: AatII - HIV-1 LTR - MCS - SV40 polyadenylation - SV40 early promoter - tat - neoR - SV40 polyadenylation - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Subramanian T, Chinnadurai G. A high-level expression vector for human cells. Gene 120: 287-289, 1992. PubMed: 1398142 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |