宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pRHLG2 [pRHLG2]

货号 TS193403
中文名称 null
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产品名称: pRHLG2 pRHLG2
商品货号: TS193403
Designations: pRHLG2 pRHLG2
GenBank Number:

M16235

Species: Rattus norvegicus, rat
Depositors: MC Schotz
Vector:
Construct size (kb): 4.639999866485596
Insert:
DNA: cDNA
Insert lengths(kb): 1.641999959945679
Tissue: liver
Gene product: lipase, hepatic LIPC
Alleles: Aa1, Bb1, Bb2, Aa2
Insert Size (kb): 1.642
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; PstI--3.4, 0.88, 0.39; SacI--3.8, 0.94.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.24, 0.61, 0.91; BstXI--0.67, 1.19; SacI--0.80.
There are corrections to the original cDNA sequence.
References:

Komaromy MC, Schotz MC. Cloning of rat hepatic lipase cDNA: evidence for a lipase gene family. Proc. Natl. Acad. Sci. USA 84: 1526-1530, 1987. PubMed: 3470738

Wong H, et al. Domain exchange: characterization of a chimeric lipase of hepatic lipase and lipoprotein lipase. Proc. Natl. Acad. Sci. USA 88: 11290-11294, 1991. PubMed: 1763042

Heinzmann C, et al. Two polymorphisms for the human hepatic lipase (HL) gene. Nucleic Acids Res. 16: 4739, 1988. PubMed: 2454457

Michael C Schotz, personal communication