| 产品名称: | pRHLG2 pRHLG2 |
|---|---|
| 商品货号: | TS193403 |
| Designations: | pRHLG2 pRHLG2 |
| GenBank Number: | M16235 |
| Species: | Rattus norvegicus, rat |
| Depositors: | MC Schotz |
| Vector: | Construct size (kb): 4.639999866485596 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.641999959945679 Tissue: liver Gene product: lipase, hepatic LIPC Alleles: Aa1, Bb1, Bb2, Aa2 |
| Insert Size (kb): | 1.642 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7; PstI--3.4, 0.88, 0.39; SacI--3.8, 0.94. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.24, 0.61, 0.91; BstXI--0.67, 1.19; SacI--0.80. There are corrections to the original cDNA sequence. |
| References: | Komaromy MC, Schotz MC. Cloning of rat hepatic lipase cDNA: evidence for a lipase gene family. Proc. Natl. Acad. Sci. USA 84: 1526-1530, 1987. PubMed: 3470738 Wong H, et al. Domain exchange: characterization of a chimeric lipase of hepatic lipase and lipoprotein lipase. Proc. Natl. Acad. Sci. USA 88: 11290-11294, 1991. PubMed: 1763042 Heinzmann C, et al. Two polymorphisms for the human hepatic lipase (HL) gene. Nucleic Acids Res. 16: 4739, 1988. PubMed: 2454457 Michael C Schotz, personal communication |