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| 产品名称： | pSluc2* Plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS193412 |
| Designations： | pSluc2* Plasmid in Escherichia coli |
| Depositors： | SK Nordeen |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.1999998092651370 Vector: pSluc2* (plasmid) Promoters: Promoter SV40 Construction: pSV232AL-Adelta5 derivative Marker(s):ampR Construct size (kb): 6.199999809265137 Features: insert detection: luciferase marker(s): ampR promoter: SV40 replicon: pMB1 terminator: none enhancer: none |
| Applications： | xa0promoter-cloning vector
xa0shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6; matches depositors DNA. There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts. This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California. One of a series of luciferase vectors (TS193412-37584) for assaying promoter activity in mammalian cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509 de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727 Steven K Nordeen, personal communication |
| Shipped： | freeze-dried |