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微信小章| 产品名称： | 44-26-2 |
|---|---|
| 商品货号： | TS193451 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized against 1D2, a BALB/cJ mouse IgG1 monoclonal antibody specific for ricin A chain. The 44-6-1 (ATCC CRL-2491) and 44-26-2 (TS193451) cell lines produce antibodies that bind specifically to an idiotypic determinant on 1D2 anti-ricin monoclonal antibody. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against an idiotypic determinant on 1D2 (anti-ricin A chain monoclonal antibody) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against an idiotypic determinant on 1D2 (anti-ricin A chain monoclonal antibody) |
| Comments： | Animals were immunized against 1D2, a BALB/cJ mouse IgG1 monoclonal antibody specific for ricin A chain. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The 44-6-1 (ATCC CRL-2491) and 44-26-2 (TS193451) cell lines produce antibodies that bind specifically to an idiotypic determinant on 1D2 anti-ricin monoclonal antibody. The 44-6-1 and 44-26-2 monoclonals conjugated to keyhole limpet hemocyanin (KLH) have been shown to induce anti-ricin antibodies in mice. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgG1 |
| Name of Depositor： | DG Sieckmann |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1995 |
| References： | Sieckmann DG, et al. Development of an anti-idiotypic vaccine for ricin. J. Allergy Clin. Immunol. 99: S286, 1997. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |