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微信小章| 产品名称： | p67-10 |
|---|---|
| 商品货号： | TS193458 |
| Designations： | p67-10 |
| GenBank Number： | M32011 |
| Species： | Homo sapiens, human |
| Depositors： | TL Leto |
| Applications： | produces protein neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) neutrophil cytosolic factor 2 |
| Vector： | Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: NCF2 Genomic copy number: unique Gene name: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 cen-q32 Tissue: HL-60 cell line (promyelocytic), differentiated Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 2.16 Cross references: DNA Seq. Acc.: M32011 Insert lengths(kb): 2.160000085830689 Tissue: HL-60 cell line (promyelocytic), differentiated Gene product: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)(neutrophil cytosolic factor 2) NCF2 Alleles: A1, A2 Target Gene: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) |
| Insert Size (kb)： | 2.160 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1; SstI--3.7, 0.9, 0.41; BamHI--4.0, 1.0; BglII--5.1; PstI--5.1. The insert contains the following restriction sites (approximate kb from the 5 end): SacI--0.84, 1.22; BamHI--0.97; BglII--0.78; SmaI--1.17. Contains the complete coding sequence (nt 68-1648). Detects a transcript of 2.4 kb. The polymorphism was detected with a 0.84 kb EcoRI/SacI fragment. Enzymes(s) not detecting polymorphism: AvaI, BamHI, EcoRI, KpnI, PstI, SacI, XhoI. |
| References： | Francke U, et al. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. PubMed: 2393022 Leto TL, et al. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60(c-src). Science 248: 727-730, 1990. PubMed: 1692159 Kenney RT, Leto TL. A HindIII polymorphism in the human NCF2 gene. Nucleic Acids Res. 18: 7193, 1990. PubMed: 1979859 |