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微信小章| 产品名称： | pUC10-41 pA10-41 |
|---|---|
| 商品货号： | TS193466 |
| Designations： | pUC10-41 pA10-41 |
| Species： | Homo sapiens, human |
| Depositors： | DF Barker |
| Vector： | Construct size (kb): 3.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.3449999988079071 Gene product: DNA Segment, single copy D17S71 Alleles: B2, A1, A2, B1 |
| Insert Size (kb)： | 0.345 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Constructed by recloning the insert from pABL10-41 along with some of the original piVX (the multiple-cloning sequence) into the EcoRI site. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.4; HindIII--2.7, 0.4; BamHI--3.1; SalI--3.1; PstI--3.1. Linked to von Recklinghausen neurofibromatosis (NF1), theta max = 0.1, LOD score = 16.9. Detects the same polymorphism as pABL10-41 (aka A10-41). Combined heterozygosity with MspI and PvuII RFLPs is about 50%. |
| References： | Baker SJ, et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244: 217-221, 1989. PubMed: 2649981 Timmerman V, et al. Assignment of the Charcot-Marie-Tooth neuropathy type I (CMT Ia) gene to 17p11.2-p12. Am. J. Hum. Genet. 47: 680-685, 1990. PubMed: 2220808 Barker D, et al. Gene for von Recklinghausen neurofibromatosis is in the pericentromeric region of chromosome 17. Science 236: 1100-1102, 1987. PubMed: 3107130 White R, et al. Tightly linked markers for the neurofibromatosis type 1 gene. Genomics 1: 364-367, 1987. PubMed: 2896632 Skolnick MH, et al. Linkage of NF1 to 12 chromosome 17 markers: a summary of eight concurrent reports. Genomics 1: 382-383, 1987. PubMed: 2896634 David F Barker, personal communication |