宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pRBC3 [pRB C3]

货号 TS193479
中文名称 null
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产品简介
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产品名称: pRBC3 pRB C3
商品货号: TS193479
Designations: pRBC3 pRB C3
GenBank Number:

D00606

Depositors: I Uyeda
Vector:
Construct size (kb): 6.199999809265137
Insert:
DNA: cDNA
Insert lengths(kb): 1.799999952316284
Gene product: genome, partial, 3 end
Insert Size (kb): 1.800
Media: ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
The insert contains 2 HindIII sites at positions 402 and 1475 of the published sequence.
Restriction digests of the clone give the following sizes (kb): PstI--4.3, 1.8; EcoRI--6.2; HindIII--4.0, 1.1, 1.0; BamHI--6.2.
This insert contains the entire 3 terminal region and lacks 16 nucleotides of the 5 terminal region. It encodes an open reading frame for 559 amino acids from nucleotide 22 to 1698.
References:

Uyeda I, et al. Nucleotide sequence of rice black-streaked dwarf virus genome segment 10. Proc. Jpn. Acad. 66: 37-40, 1990.

Azuhata F, et al. Characterization of the cDNA clone to rice black-streaked dwarf virus genome segment 10. J. Fac. Agric. Hokkaido Univ. 64: 183-189, 1990.