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| 产品名称： | HepG2-16 HepG2.16 |
|---|---|
| 商品货号： | TS193555 |
| Designations： | HepG2-16 HepG2.16 |
| Species： | Homo sapiens, human |
| Depositors： | P Borst |
| Applications： | In H4638 and H7325, a 0.45 HindII/PstI fragment was used as a probe. |
| Vector： | Name of vector: pcD Construct size (kb): 5.099 Intact vector size: 3.000 Type of vector: plasmid Cloning sites: XhoI BamHI Polylinker sites: Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: SV40 marker(s): ampR promoter: SV40 early, late replicon: pMB1, SV40 terminator: SV40 late Cross references: |
| Insert： | Genome: Homo sapiens Gene symbol: PGY3 Other gene symbols: MDR3 Genomic copy number: unique Gene name: P glycoprotein 3/multiple drug resistance 3 Contains complete coding sequence?: U Chromosome: 7 Localization: 7 q21 Tissue: hepatocellular carcinoma Hep G2 cell line Type of DNA: cDNA Insert 5 end: Modification: GC Insert 3 end: Modification: AT Insert size (kb): 2.0 Cross references: |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.05, 1.9; SalI--4.9; HindIII--4.9; ClaI--4.9; EcoRI--4.9. The cDNA insert can be released using the flanking BamHI sites from the SV40 sequences in the vector. The insert contains HindII, EcoRI, and PstI (2) sites. The HindII/PstI fragment encoding the transmembrane segment is sufficiently divergent to distinguish between mdr1 and mdr3. This variation of mdr3 cDNA encodes a 7 amino acid, Phe-rich insertion between the putative nucleotide binding sites. In H4638 and H7325, a 0.45 HindII/PstI fragment was used as a probe. |
| References： | Van der Bliek AM, et al. The human mdr3 gene encodes a novel p-glycoprotein homologue and gives rise to alternatively spliced mRNAs in liver. EMBO J. 6: 3325-3331, 1987. PubMed: 2892668 |